Methods for genetic transformation of filamentous fungi
نویسندگان
چکیده
منابع مشابه
Methods for genetic transformation of filamentous fungi
Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking ...
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Transformation systems have been reported for a number of filamentous fungi (1) based on the procedure perfected for Neurospora crassa by Case et al. employing sphaeroplasts (2). While efficient transformation is achieved with sphaeroplasts, their preparation demands a careful monitoring of the individual steps, along with optimization of conditions for each batch of cell wall-degrading enzymes...
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The vast majority of transformation protocols for filamentous fungi are based on permeabilizing cell membranes with polyethylene glycol (PEG) or electroporation. Both methods typically require preparing protoplasts or osmotically sensitive cells prior to transformation (Herzog et al., 1996; Goldman et al., 1990; a detailed review of fungal transformation systems is given by Lemke and Peng, 1995...
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The number of fully sequenced fungal genomes is rapidly increasing. Since genetic tools are poorly developed for most filamentous fungi, it is currently difficult to employ genetic engineering for understanding the biology of these fungi and to fully exploit them industrially. For that reason there is a demand for developing versatile methods that can be used to genetically manipulate non-model...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2017
ISSN: 1475-2859
DOI: 10.1186/s12934-017-0785-7